Revision as of 17:32, 11 August 2009 by >Jie deng
breast cancer patient peripheral blood DNA sample capture by cpg97k
I used the Qiagen FlexiGene DNA kit to exact the DNA from blood. yield:
05192A18: 83.5ng/ul x 100ul;
05192B09: 72.7ng/ul x 100ul
Bisulfite conversion of patient DNA
No
|
sample
|
sample concentration
|
sample volumn
|
ddH2O
|
conversion reagents
|
conversed DNA concentration and volumn
|
|
05192A18 |
83.5ng/ul x 1 tube |
20ul |
0ul |
130ul |
148.2ng/ul x 10ul
|
|
05192B09 |
72.7ng/ulx 1 tubes |
20ul |
0ul |
130ul |
127.8ng/ul x 10ul
|
cpature by cpg97k
}
No
|
sample
|
sample concentration
|
10xLigase buffer
|
template+cpg97k_A(25.6ng/ul_06/18) vol+suppress oligo+H2O
|
template+cpg97k_B(21ng/ul_06/18) vol+suppressor oligo+H2O
|
|
05192A18 |
148.2ng/ul |
1ul |
4+2.5ul+1ul+1.5ul |
4+2ul+1ul+2ul
|
|
05192B09 |
127.8ng/ul |
1ul |
4+2.5ul+1ul+1.5ul |
4+2ul+1ul+2ul
|