Jie:LabNotes/CpgSeq/2009-8-7

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Revision as of 17:32, 11 August 2009 by >Jie deng
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breast cancer patient peripheral blood DNA sample capture by cpg97k

gDNA extraction from blood

I used the Qiagen FlexiGene DNA kit to exact the DNA from blood. yield:
05192A18: 83.5ng/ul x 100ul;
05192B09: 72.7ng/ul x 100ul

Bisulfite conversion of patient DNA

No sample sample concentration sample volumn ddH2O conversion reagents conversed DNA concentration and volumn
05192A18 83.5ng/ul x 1 tube 20ul 0ul 130ul 148.2ng/ul x 10ul
05192B09 72.7ng/ulx 1 tubes 20ul 0ul 130ul 127.8ng/ul x 10ul

cpature by cpg97k

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No sample sample concentration 10xLigase buffer template+cpg97k_A(25.6ng/ul_06/18) vol+suppress oligo+H2O template+cpg97k_B(21ng/ul_06/18) vol+suppressor oligo+H2O
05192A18 148.2ng/ul 1ul 4+2.5ul+1ul+1.5ul 4+2ul+1ul+2ul
05192B09 127.8ng/ul 1ul 4+2.5ul+1ul+1.5ul 4+2ul+1ul+2ul