Kun:LabNotes/ASE/2008-1-29

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Exp. 2 PCR on the CES27k capturing reactions started on 01/27/2008[edit]

                          NA20431 GM20431  Hues36g   Hues36c 
    Template:              10ul     10ul     10ul     10ul   
    10X JumpStart buffer:   9ul      9ul      9ul      9ul
    10mM dNTP               2ul      2ul      2ul      2ul 
    100uM AmpFV6.2Sol     0.4ul    0.4ul    0.4ul    0.4ul  
    100uM AmpRV6.2Sol     0.4ul    0.4ul    0.4ul    0.4ul 
    50X SYBG I            0.4ul    0.4ul    0.4ul    0.4ul
    JumpStart Taq           2ul      2ul      2ul      2ul  
    H2O                    76ul     76ul     76ul     76ul 

94C 3min -> (94C 45sec -> 56C 1min -> 72C 1min) x 8 -> (94C 30sec -> 72C 30sec) x 10 -> 72C 3min -> 4C hold.

 File:2008-01-29-CES27k-capture-PCR.png