AlanFung:LabNotes/EZ/2009-8-14

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Revision as of 19:25, 14 August 2009 by >Alan6017518 (→‎Objective)
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EZ DNA Methylation Direct - GM20431 cells

Objective

  • Extract DNA from 2000 GM20431 cells
  • Elute DNA with 8uL elution buffer
  • Use all 8uL dna as template for one pair of primer
  • Quantify amount of DNA with Nanodrop Qubit and Gel quantification
  • Perform serial dilution to obtain
200ng methylated DNA
100ng methylated DNA
50ng methylated DNA
30ng methylated DNA
10ng methylated DNA