AlanFung:LabNotes/EZ/2009-8-14
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EZ DNA Methylation Direct - GM20431 cells[edit]
Objective[edit]
- Extract DNA from 2000 GM20431 cells
- Elute DNA with 8uL elution buffer
- Use all 8uL dna as template for one pair of primer
- Quantify amount of DNA with Nanodrop Qubit and Gel quantification
- Perform serial dilution to obtain
200ng methylated DNA |
100ng methylated DNA |
50ng methylated DNA |
30ng methylated DNA |
10ng methylated DNA |
' | 2000 cells | 100,000 cells |
M-Digestion Buffer | 10ul | 13ul |
Sample | up to 9ul | up to 12ul |
Proteinase K | 1ul | 1ul |
H20 | X ul | x ul |
Total Volume | 20ul | 26 ul |