AlanFung:LabNotes/DNA/2009-8-18
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Digestion with MmeI
No_1_2_3_9 x4 Total 30ul dUTP_PCR 20ul 10X NEBuffer 4 4ul 16 1mM SAM(fresh) 4ul 16 2U/ul Mme I 8ul 32 ddH2O 4ul 16 1mM SAM: 32mM SAM 1ul + 31ul ddH2O. 37C 2h. MinEulte column purification. Elute in 12ul EB.