AlanFung:LabNotes/DNA/2009-8-18

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Revision as of 18:42, 18 August 2009 by >Alan6017518 (New page: ==Digestion with MmeI== No_1_2_3_9 x4 Total 30ul dUTP_PCR 20ul ...)
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Digestion with MmeI

                                     No_1_2_3_9    x4 
 Total                                30ul                  
 dUTP_PCR                             20ul         
 10X NEBuffer 4                       4ul          16           
 1mM SAM(fresh)                       4ul          16             
 2U/ul Mme I                          8ul          32               
 ddH2O                                4ul          16
 
 1mM SAM: 32mM SAM 1ul + 31ul ddH2O.
 37C 2h. MinEulte column purification. Elute in 12ul EB.