AlanFung:LabNotes/DNA/2009-8-18

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Revision as of 19:19, 18 August 2009 by >Alan6017518 (→‎Digestion with MmeI)
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Digestion with MmeI

' PGP4 PGP6 PGP10 A B
Sample 20 20 20 10 10
10X NEBuffer 4 4 4 4 3 3
1mM SAM (fresh) 4 4 4 3 3
2U/ul MmeI 1 1 1 1 1
ddH20 11 11 11 13 13
Total 40 40 40 30 30
 1mM SAM: 32mM SAM 1ul + 31ul ddH2O.
 37C 2h. MinEulte column purification. Elute in 12ul EB.