AlanFung:LabNotes/DNA/2009-8-18
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Digestion with MmeI
' | PGP4 | PGP6 | PGP10 | A | B |
Sample | 20 | 20 | 20 | 10 | 10 |
10X NEBuffer 4 | 4 | 4 | 4 | 3 | 3 |
1mM SAM (fresh) | 4 | 4 | 4 | 3 | 3 |
2U/ul MmeI | 1 | 1 | 1 | 1 | 1 |
ddH20 | 11 | 11 | 11 | 13 | 13 |
Total | 40 | 40 | 40 | 30 | 30 |
1mM SAM: 32mM SAM 1ul + 31ul ddH2O. 37C 2h.
- Qiaquick column purification. Elute in 12ul EB.