AlanFung:LabNotes/DNA/2009-8-18

From ZhangLabWiki
Revision as of 19:26, 18 August 2009 by >Alan6017518 (→‎Digestion with MmeI)
Jump to navigation Jump to search

Digestion with MmeI

PGP4 PGP6 PGP10 A B
Sample 20 20 20 10 10
10X NEBuffer 4 4 4 4 3 3
1mM SAM (fresh) 8 8 8 6 6
2U/ul MmeI 1 1 1 1 1
ddH20 7 7 7 10 10
Total 40 40 40 30 30
 1mM SAM: 32mM SAM 1ul + 31ul ddH2O.
 37C 2h. 
  • Qiaquick column purification. Elute in 12ul EB.