Athurva Gore/LabNotes/2009-8-24

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Revision as of 17:07, 24 August 2009 by >Ajgore (→‎Probe Sets)
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Probe Sets

  • FLYDUP5279 has finished; all old probesets are done
  • Need to create and send invoice to Zymo today
    • Also mention that probe generation can be rerun without quality filters?
  • Need to redo TDMR set and Cpg-SNP set, but with a new probe design...
    • Constrain arm+target size to 105 bp
    • Then modify P2MIP_v9 to use a 45 bp total arm size
    • This will allow library construction to not be necessary
      • During capture, MME1 digestion removes 17-18 bp from each side of H1_TARGET_H2
      • Should ideally leave us with 75 bp segment remaining, 60 bp of which will be target
  • This can be read in the sequencer without library construction, allowing for a much quicker and more efficient experimental procedure