Jeff:LabNotes/Microbiome/2009-8-27

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Revision as of 18:41, 27 August 2009 by >Jgole (New page: == '''Test to see if DNA attaches to bead: SYBR Green Staining''' == ''Before we try and hybridize, I want to ensure that amplified DNA is attached to the bead. '' *Use product from MD...)
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Test to see if DNA attaches to bead: SYBR Green Staining[edit]

Before we try and hybridize, I want to ensure that amplified DNA is attached to the bead.

  • Use product from MDA on 8-26-09
  • Place PCR tube next to magnet, let beads assemble, and remove agarose gel
  • Add 20 uL of PBS and mix with pipette
  • Add 1 uL of 50X SYBR Green and mix
  • Incubate at RT in dark for 5 min
  • Pipette 5 uL onto a slide to view on a microscope

Results

The following were taken with a green filter. Product was observed, showing that the DNA was attached to the beads.

File:8-27-09 1.jpeg 20X mag

File:8-27-09 2.jpeg 10X mag

File:8-27-09 3.jpeg 50X mag