Jeff:LabNotes/Microbiome/2009-8-27
Jump to navigation
Jump to search
Test to see if DNA attaches to bead: SYBR Green Staining[edit]
Before we try and hybridize, I want to ensure that amplified DNA is attached to the bead.
- Use product from MDA on 8-26-09
- Place PCR tube next to magnet, let beads assemble, and remove agarose gel
- Add 20 uL of PBS and mix with pipette
- Add 1 uL of 50X SYBR Green and mix
- Incubate at RT in dark for 5 min
- Pipette 5 uL onto a slide to view on a microscope
Results
The following were taken with a green filter. Product was observed, showing that the DNA was attached to the beads.
File:8-27-09 1.jpeg 20X mag
File:8-27-09 2.jpeg 10X mag
File:8-27-09 3.jpeg 50X mag