August Library (8/24/09)

From ZhangLabWiki
Revision as of 03:40, 14 September 2009 by >Jbrubake
Jump to navigation Jump to search

Probes, Samples, and Calculations

Probes:

Jan09-1:  12.4 ng/ul,  46417 probes;  376 nM
Jan09-2:  16.3 ng/ul,  53493 probes;  494 nM
Jan09-3:  7.8 ng/ul,   53828 probes;  236 nM

Samples:

Sample #	        Sample name	       Amount	     Concentration
  1	            MH001 1+2 xHeaIII	       10 ul	      102.7 ng/ul
  2	                 MH010B xMboI	        6 ul	      183.4 ng/ul
  3	                 MH010B xDpnI	       10 ul  	      100 ng/ul
  4	                   TCF #3	        6 ul	      200 ng/ul
  5                        DK4 #2	        17 ul	      58.9 ng/ul
  6	                   TK4 #2	        18 ul	      55.9 ng/ul
  7	                   TK9	                12 ul	      89.4 ng/ul 
  8	                   Pol II	        10 ul	      99.7 ng/ul

Calculations

Probe: Target ratio is 100:1
The mass of one genome is 3pg: 200ng of DNA is equal to 200ng/3pg.
The amount of probe needed is 200ng/3pg * 250 for each target, and the total amount of probe is 
(200ng/3pg * 100 * 46417)/(6.0 * 10^23)

Example
Assume the concentration of probe Jan09-1 to be 10ng/ul. Knowing the length of the probe is ~100 bases and 1 base = 330 Daltons (1bp = 2 bases = 660 Da).
Solve: Concentration of Jan09-1 probe: 10ng/ul / 33000 = 303 nM
    	Volume of probe needed= 
(200ng/3pg * 100 * 46417)/(6.0 * 10^23 * 303 nM) = 1.73ul