Jeff:LabNotes/Microbiome/2009-9-23
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Extraction of 500 bp plasmid insertion
- The biomiga miniprep II kit was used for extraction
- The product was run on a 1 % agarose gel for 40 min
- No restriction was used yet since it needs to be ordered
Results
The gel shows a similar band as the previous gel. Thus, a restriction enzyme must be used in order for the DNA to properly move through the gel.