AlanFung:LabNotes/Cell/2009-10-11

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Revision as of 18:15, 12 October 2009 by >Alan6017518 (→‎H9)
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MEF

  • Make 3x10cm MEF plates for HUES MCM
  • Make 3x10cm MEF plates for Wicell MCM

HUES9

  • Feed 1x10cm (matrigel) + 1 well (mef) w/ 20% HUES MCM

H9

HUES53

  • Cells are ~80% confluent, split 1x10cm (matrigel) (1:2) to 2x10cm (matrigel) w/100% HUES MCM
  • Feed 3 wells (mef) w/ 20% HUES MCM
  • Cells are probably ready to collect on Monday

Matrigel