Baylor-exon-Sept09

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Revision as of 02:14, 4 November 2009 by >Jbrubake
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Baylor (BCM) Creation

PCR

                                          1x               48x

Template 0.4ul 19.2ul 2x Kapa Master Mix 50ul 50ul H2O 50ul 50ul eMIP-CA1F (100uM) 0.4ul 0.4ul eMIP-CA1R (100uM) 0.4ul 0.4ul PCR: 95C, 30sec -> (95C, 3sec -> 54C, 30sec -> 60C, 20sec -> plate read) x 25 -> 60C, 2min -> 15C hold

  • Note: Amplification started after ~13 cycles