Baylor-exon-Sept09

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Revision as of 02:15, 4 November 2009 by >Jbrubake (→‎Baylor (BCM) Creation)
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Baylor (BCM) Creation

PCR

                                           1x               48x
Template                         0.4ul         19.2ul 
2x Kapa Master Mix       50ul             50ul
H2O                                  50ul             50ul
eMIP-CA1F (100uM)     0.4ul           0.4ul
eMIP-CA1R (100uM)     0.4ul          0.4ul
PCR: 95C, 30sec -> (95C, 3sec -> 54C, 30sec -> 60C, 20sec -> plate read) x 25 -> 60C, 2min -> 15C hold
  • Note: Amplification started after ~13 cycles