AlanFung:LabNotes/Sequencing/2009-11-11
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Rescue CVF-N
- 20ul of CVF-N library, with buffer added instead of iProof master mix
Ethanol Precipitation
- Mix 50ul 100% ethanol, 2ul 3M NaoAC, and 1ul glycoblue
- Store at -80C for 20min
- Centrifuge 10,000 for 20min at 4C
- Discard supernatant
- Add in 750ul 75%ethanol
- Spin 10,000 rpm for 5 min at 4C
- Discard supernatant
- let dry in hood for 10 min
- Add in 21ul ddh2o
Repeat PCR of sequencing library with the following sets