AlanFung:LabNotes/Sequencing/2009-11-19
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Quantification of New amplified Phi-X and all libraries
Samples | ||||
PhiX-Stock | CVF | PGP4 | PGP10 | PGP8C |
PhiX-Stock | CVF | PGP4 | PGP10 | PGP8C |
PhiX | 1-7 PGP1 | PGP6 | NA12878 | PGP8D |
PhiX | 1-7 PGP1 | PGP6 | NA12878 | PGP8D |
PhiX | 8 PGP1 | PGP8 | PGP8A | PGP8E |
PhiX | 8 PGP1 | PGP8 | PGP8A | PGP8E |
CVI | 9 PGP1 | PGP9 | PGP8B | N12 |
CVI | 9 PGP1 | PGP9 | PGP8B | N12 |
X20 | X1.1 | ||
PhiX Control DNA (10nM) | 1.000 | ||
Syb_FP5 (100uM) | 0.200 | 4.000 | 4.400 |
Syb_RP7 (100uM) | 0.200 | 4.000 | 4.400 |
2X Phusion | 25.000 | 500.000 | 550.000 |
50X SYBR Green I | 0.200 | 4.000 | 4.400 |
H20 | 24.000 | 480.000 | 528.000 |