Revision as of 18:38, 19 November 2009 by >Alan6017518
Quantification of New amplified Phi-X and all libraries
- Dilute all samples by 10 fold
Samples |
|
|
|
|
PhiX-Stock |
CVF |
PGP4 |
PGP10 |
PGP8C
|
PhiX-Stock |
CVF |
PGP4 |
PGP10 |
PGP8C
|
PhiX-Stock |
1-7 PGP1 |
PGP6 |
NA12878 |
PGP8D
|
PhiX-Stock |
1-7 PGP1 |
PGP6 |
NA12878 |
PGP8D
|
PhiX |
8 PGP1 |
PGP8 |
PGP8A |
PGP8E
|
PhiX |
8 PGP1 |
PGP8 |
PGP8A |
PGP8E
|
CVI |
9 PGP1 |
PGP9 |
PGP8B |
N12
|
CVI |
9 PGP1 |
PGP9 |
PGP8B |
N12
|
|
- split every 50ul rxn into 2 wells
98C 30 sec -> 20 cycles of (98C 10sec->64C 20sec->72C 20sec)->72C 5 min
|
|
X20 |
X1.1
|
PhiX Control DNA (10nM) |
1.000 |
|
|
Syb_FP5 (100uM) |
0.200 |
4.000 |
4.400
|
Syb_RP7 (100uM) |
0.200 |
4.000 |
4.400
|
2X Phusion |
25.000 |
500.000 |
550.000
|
50X SYBR Green I |
0.200 |
4.000 |
4.400
|
H20 |
24.000 |
480.000 |
528.000
|
|