Enzyme Test

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Revision as of 22:22, 20 November 2009 by >Jbrubake (New page: *Both rounds of Nicking Enzymes, used on both BCM sets, failed to properly digest the DNA. In order to get a better understanding of the situation, the BCM set was re-amplified and the two...)
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  • Both rounds of Nicking Enzymes, used on both BCM sets, failed to properly digest the DNA. In order to get a better understanding of the situation, the BCM set was re-amplified and the two enzymes, Nt.A1wI and Nb.BsrDI were tested as follows:

BCM Preparation

Nt.A1wI