AlanFung:LabNotes/Sequencing/2009-11-22
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CVF-N
- Reamplify library and ship it out for shearing
' | ' | ' | x4 | X1.1 |
Library | 12 | 0.25 | 1 | 1.1 |
2X Phusion | 50 | 50 | 200 | 220 |
AmpF6.3NH2 (10uM) | 2 | 2 | 8 | 8.8 |
AmpR6.3NH2 (10uM) | 2 | 2 | 8 | 8.8 |
10X SYBR Green I | 2 | 2 | 8 | 8.8 |
dH2O | 32 | 43.75 | 175 | 192.5 |
- 98C 1min->6X(98C 10 sec, 58C 20 sec, 72C 20sec)->72C 5 min