AlanFung:LabNotes/Sequencing/2009-11-22
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CVF-N
- Reamplify library and ship it out for shearing
' | ' | x4 | X1.1 | |
Library | 1 | - | - | - |
2X Phusion | 50 | 750 | 825 | |
AmpF6.3NH2 (100uM) | 2 | 3 | 3.3 | |
AmpR6.3NH2 (100uM) | 2 | 3 | 3.3 | |
50X SYBR Green I | 2 | 3 | 3.3 | |
dH2O | 32 | 645 | 709.5 | |
- 98C 1min->6X(98C 10 sec, 58C 20 sec, 72C 20sec)->72C 5 min