AlanFung:LabNotes/Sequencing/2009-11-22

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Revision as of 05:38, 23 November 2009 by >Alan6017518 (→‎CVF-N)
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CVF-N

  • Reamplify library and ship it out for shearing
' ' x15 X1.1 /2
Library 1 - - -
2X Phusion 50 750 825 411.5
AmpF6.3NH2 (100uM) 0.2 3 3.3 1.65
AmpR6.3NH2 (100uM) 0.2 3 3.3 1.65
50X SYBR Green I 0.2 3 3.3 1.65
dH2O 43 645 709.5 354.75
  • 98C 1min->6X(98C 10 sec, 58C 20 sec, 72C 20sec)->72C 5 min