AlanFung:LabNotes/Sequencing/2009-11-27

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Revision as of 06:35, 27 November 2009 by >Alan6017518
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  • Ethanol Precipitation
  • Dissolve all in 14ul ddH2O

QPCR Quantification

Setup
Stock Phi-X (1nM)   CVF-N (Shearing) (1/10)
Stock Phi-X (1nM)   CVF-N (Shearing) (1/10)
Stock Phi-X (1nM)   CVF-N (Shearing) (1/10)
Stock Phi-X (1nM)   CVF-N (Shearing) (1/10)
Amp Phi-X (1nM)     CVF-N (A/B) (1/10)
Amp Phi-X (1nM)     CVF-N (A/B) (1/10)
Amp Phi-X (1nM)     CVF-N (A/B) (1/10)
Amp Phi-X (1nM)     CVF-N (A/B) (1/10)
X8 X1.1
DNA 1.000
Syb_FP5 (100uM) 0.200 1.600 1.760
Syb_RP7 (100uM) 0.200 1.600 1.760
2X Phusion 25.000 200.000 220.000
50X SYBR Green I 0.200 1.600 1.760
dH2O 24.000 192.000 211.200