Revision as of 07:05, 27 November 2009 by >Alan6017518
- Ethanol Precipitation
- Dissolve all in 14ul ddH2O
QPCR Quantification[edit]
Setup
Stock Phi-X (1nM) CVF-N (Shearing) (1/10)
Stock Phi-X (1nM) CVF-N (Shearing) (1/10)
Stock Phi-X (1nM) CVF-N (Shearing) (1/10)
Stock Phi-X (1nM) CVF-N (Shearing) (1/10)
Amp Phi-X (1nM) CVF-N (A/B) (1/10)
Amp Phi-X (1nM) CVF-N (A/B) (1/10)
Amp Phi-X (1nM) CVF-N (A/B) (1/10)
Amp Phi-X (1nM) CVF-N (A/B) (1/10)
|
|
X8 |
X1.1
|
DNA |
1.000 |
|
|
Syb_FP5 (100uM) |
0.200 |
1.600 |
1.760
|
Syb_RP7 (100uM) |
0.200 |
1.600 |
1.760
|
2X Phusion |
25.000 |
200.000 |
220.000
|
50X SYBR Green I |
0.200 |
1.600 |
1.760
|
dH2O |
24.000 |
192.000 |
211.200
|
|
- Dilute sample, 1ul in 9ul ddh2o
- mix 2ul 1/10 sample with 98ul mix
- aliquot 24ul to each well
98C 30 sec -> 20 cycles of (98C 10sec->64C 20sec->72C 20sec)->72C 5 min
|
|
Sum |
Ave |
Diff |
2^ |
X10
|
CVF-N-S |
8.162 |
24.091 |
8.030 |
2.819 |
7.057 |
70.567
|
0.070 |
8.040 |
|
|
|
|
|
|
7.889 |
|
|
|
|
|
PhiX Stock |
11.020 |
32.548 |
10.849 |
|
|
|
0.070 |
10.883 |
|
|
|
|
|
|
10.645 |
|
|
|
|
|
|
|
|
|
|
|
|
CVF-N A/B |
7.850 |
32.161 |
8.040 |
1.791 |
3.460 |
34.599
|
0.050 |
8.002 |
|
|
|
|
|
|
8.146 |
|
|
|
|
|
|
8.163 |
|
|
|
|
|
PhiX Stock |
9.665 |
29.493 |
9.831 |
|
|
|
0.050 |
9.876 |
|
|
|
|
|
|
9.952 |
|
|
|
|
|
|