AlanFung:LabNotes/Capturing/BisFirstExon 2009 12 31
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PCR Amplification of Captured product
1 well (uL) | X 6 | 1 well (uL) | X 6 | |||
Template | 10 | Template | ||||
2X iProof Mastermix | 50 | 300 | 2X iProof Mastermix | 50 | 300 | |
Amp F6.3 Sol (100uM) | 0.4 | 2.4 | Amp F6.3r Sol (100uM) | 0.4 | 2.4 | |
Amp R6.3Sol (100uM) | 0.4 | 2.4 | Amp R6.3r Sol (100uM) | 0.4 | 2.4 | |
50X SYBG I | 0.8 | 4.8 | 50X SYBG I | 0.8 | 4.8 | |
H2O | 31.2 | 187.2 | H2O | 31.2 | 187.2 | |
Total Volume | 90 | 540 | Total Volume | 90 | 540 | |
98C 30S -> (98C 10S -> 60C 20S -> 72C 20S) x 8 -> (98C 10S -> 72C 20S) x 20-> 72C 5 min -> 15C hold