AlanFung:LabNotes/Capturing/BisFirstExon 2009 12 31

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PCR Amplification of Captured product

NA12878 NA18507 NA20431 Jurkat New Jurkat Old

1 well (uL) X 6 1 well (uL) X 6
Template 10 Template
2X iProof Mastermix 50 300 2X iProof Mastermix 50 300
Amp F6.3 Sol (100uM) 0.4 2.4 Amp F6.3r Sol (100uM) 0.4 2.4
Amp R6.3Sol (100uM) 0.4 2.4 Amp R6.3r Sol (100uM) 0.4 2.4
50X SYBG I 0.8 4.8 50X SYBG I 0.8 4.8
H2O 31.2 187.2 H2O 31.2 187.2
Total Volume 90 540 Total Volume 90 540

98C 30S -> (98C 10S -> 60C 20S -> 72C 20S) x 8 -> (98C 10S -> 72C 20S) x 20-> 72C 5 min -> 15C hold

TBE Gel