Capture Test of CES36k18bp

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Revision as of 23:18, 5 January 2010 by >Jbrubake
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Capture Protocol

Probe and Sample

Probes: CES36k18bp, 5.6ng/ul

Sample is Jurkat DNA standard, 100ng/ul

The Probe:Target ratio is 100:1

Calculations

Probe:

CES36k18bp: 5.6ng/ul;36,000 probes; 170nM
Volume of CES36k18bp needed = (200ng/3pg * 100 * 36000)/(6.0 * 10^23 * 170 nM) = 2.35ul

Reaction Setup