Revision as of 16:28, 15 January 2010 by >Alan6017518
Contents
- 1 Quantify Bisulfite Converted Samples with Qubit ssDNA Kit
- 2 On 50ng, 10ng, 5ng, 1ng, 0.5ng, 0.1ng & 0.05ng P21-200BP
- 3 PCR quantification of Bisulfite treated samples using 3 paris of CHR bilsulfite conversion primers
- 4 On 50ng, 10ng, 5ng, 1ng, 0.5ng, 0.1ng & 0.05ng P21-200BP
Quantify Bisulfite Converted Samples with Qubit ssDNA Kit
On 50ng, 10ng, 5ng, 1ng, 0.5ng, 0.1ng & 0.05ng P21-200BP
7 samples
Prepare 7 Qubit Assay tubes
Prepare 7*200ul diluted dye=1400ul
Mix 7ul dye with 1393ul buffer
Mix 2ul samples with 98ul diluted dye
vortex spin down and wait for 2 mins
Result
PCR quantification of Bisulfite treated samples using 3 paris of CHR bilsulfite conversion primers
On 50ng, 10ng, 5ng, 1ng, 0.5ng, 0.1ng & 0.05ng P21-200BP