AlanFung:LabNotes/CTCF/2010-1-15

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Quantify Bisulfite Converted Samples with Qubit ssDNA Kit

On 50ng, 10ng, 5ng, 1ng, 0.5ng, 0.1ng & 0.05ng P21-200BP

7 samples
Prepare 7 Qubit Assay tubes

Prepare 7*200ul diluted dye=1400ul
Mix 7ul dye with 1393ul buffer
Mix 2ul samples with 98ul diluted dye
vortex spin down and wait for 2 mins

Result
Start (ng) Sample Concentration ng/ul Volume Amount Recovered (ng) Yield %
50 1.7 20 34 68
10 0.25 20 5 50
5 0.13 20 2.6 52
1 0.26 20 5.2 520
0.5 N/A 20
0.1 N/A 20
0.05 N/A 20

PCR quantification of Bisulfite treated samples using 3 paris of CHR bilsulfite conversion primers

On 50ng, 10ng, 5ng, 1ng, 0.5ng, 0.1ng & 0.05ng P21-200BP