Calendar/2010-1-16
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Quantify libraries with Phi-X ladder qPCR
AL-Exome-CViB-Jan14-AB w/ 1/10 dilution, AL-Exome-CViB-Jan14-CDE w/ 1/10 dilution, Ida's library
Setup | ||
1nm PhiX | AL-Exome-CViB-Jan14-AB | AL-Exome-CViB-Jan14-CDE |
1nm PhiX | AL-Exome-CViB-Jan14-AB | AL-Exome-CViB-Jan14-CDE |
1nm PhiX | AL-Exome-CViB-Jan14-AB | AL-Exome-CViB-Jan14-CDE |
1nm PhiX | AL-Exome-CViB-Jan14-AB | AL-Exome-CViB-Jan14-CDE |
Ida | 1/10 AL-Exome-CViB-Jan14-AB | 1/10 AL-Exome-CViB-Jan14-CDE |
Ida | 1/10 AL-Exome-CViB-Jan14-AB | 1/10 AL-Exome-CViB-Jan14-CDE |
Ida | 1/10 AL-Exome-CViB-Jan14-AB | 1/10 AL-Exome-CViB-Jan14-CDE |
Ida | 1/10 AL-Exome-CViB-Jan14-AB | 1/10 AL-Exome-CViB-Jan14-CDE |
2 Wells | X13 | |
DNA | 2 | 26 |
Syb_FP5 (100uM) | 0.2 | 2.6 |
Syb_RP7 (100uM) | 0.2 | 2.6 |
2X Phusion | 25 | , |
50X SYBR Green | 0.2 | 2.6 |
H2O | 24 | 312 |
Total | 345.8 | |
* Dilute sample, 1ul in 9ul ddh2o * mix 2ul 1/10 sample with 98ul mix in non-stick tube * aliquot 24ul to each well, for each sample use the same tip for every well (Pipet slowly!)
98C 30 sec -> 20 cycles of (98C 10sec->64C 20sec->72C 20sec)->72C 5 min
Media:JD-cpg97k+30k-Breast_cancer-01230B09-Jan15_log.pdf Media:AL-Exome-CViB-Jan14-AB.pdf Media:AL-Exome-CViB-Jan14-CDE.pdf