AlanFung:Protocol/KAPA Library Quantification Kits-GA

From ZhangLabWiki
Revision as of 18:53, 20 January 2010 by >Alan6017518 (Created page with '==Prepare qPCR Reagents== ===Ensure that the following reagents are completely thawed and thoroughly mixed by vortexing=== *2X KAPA SYBR FAST qPCR Master Mix *6 DNA Standards *1:…')
(diff) ← Older revision | Latest revision (diff) | Newer revision → (diff)
Jump to navigation Jump to search

Prepare qPCR Reagents

Ensure that the following reagents are completely thawed and thoroughly mixed by vortexing

  • 2X KAPA SYBR FAST qPCR Master Mix
  • 6 DNA Standards
  • 1:1000, 1:2000, 1:4000 & 1:8000 dilution of library DNA

Step1

  • Perform an an 1:1000 dilution of the purified library in Library Dilution Buffer
  • Optional 1:2000, 1:4000, 1:8000

Step2