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Quantify libraries with Phi-X ladder qPCR
Stock Phi-X (1nM),Stock Phi-X (0.1nM), Stock Phi-X (0.05nM), JD-cpg97k+30k-Breast_cancer-01230B09-Jan15,1/10 JD-cpg97k+30k-Breast_cancer-01230B09-Jan15, 1/10 AL-Exome-CViB-Jan14-AB, 1/100 AL-Exome-CViB-Jan14-AB, 1/10 AL-Exome-CViB-Jan14-CDE, 1/100 AL-Exome-CViB-Jan14-CDE
Setup | ||
Stock Phi-X (1nM) | 1/10 JD-cpg97k+30k-Breast_cancer-01230B09-Jan15 | 1/100 AL-Exome-CViB-Jan14-CDE |
Stock Phi-X (1nM) | 1/10JD-cpg97k+30k-Breast_cancer-01230B09-Jan15 | 1/100 AL-Exome-CViB-Jan14-CDE |
Stock Phi-X (0.1nM) | 1/10 AL-Exome-CViB-Jan14-AB | |
Stock Phi-X (0.1nM) | 1/10 AL-Exome-CViB-Jan14-AB | |
Stock Phi-X (0.05nM) | 1/100 AL-Exome-CViB-Jan14-AB | |
Stock Phi-X (0.05nM) | 1/100 AL-Exome-CViB-Jan14-AB | |
For 2 wells | X10 | |
DNA | 2.000 | |
Syb_FP5 (100uM) | 0.200 | 2.000 |
Syb_RP7 (100uM) | 0.200 | 2.000 |
2X Phusion | 25.000 | 250.000 |
50X SYBR Green I | 0.200 | 2.000 |
H20 | 24.000 | 240.000 |