Protease and Proteinase Trials
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Two different denaturants were used:
- Protease (Qiagen) - abbreviated P.
- Proteinase K (Invitrogen) - abbreviated P.K.
Solution Preparations
The protease (Qiagen) was easily reconstituted: 7uL water per vial
See Proteinase K Protocol for preparation of Proteinase K (Invitrogen)
3/31/10
2 columns/strips of sorted chromosomes were collected from Sam. The strips were separated as follows:
| H | - Head
Row 1 | o | - No chromosomes Row 2 | o | - 1 chromosome Row 3 | o | - 12 chromosomes Row 4 | o | - 12 chromosomes Row 5 | o | - 12 chromosomes Row 6 | o | - 23 chromosomes Row 7 | o | - 23 chromosomes Row 8 | o | - 23 chromosomes
| A | - Tail