PrepGEM (ZyGEM) Preparation
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Preparation[edit]
Preparing the PrepGEM denaturant is very simple:
Per reaction: - 1uL 10X Gold Buffer - 0.9uL water - 0.1uL PrepGEM substrate
---Revised (as of 4/30/10)---
Per reaction: - 0.1uL 10X Gold Buffer - 0.89uL water - 0.01uL PrepGEM substrate
Note: In order to make a reasonable mixture, enough volume for at least 10 reactions should be mixed at once and split up as much as needed.