PrepGEM (ZyGEM) Preparation

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Revision as of 20:52, 3 May 2010 by >Jbrubake (→‎Preparation)
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Preparation[edit]

Preparing the PrepGEM denaturant is very simple:

Per reaction:
- 1uL 10X Gold Buffer
- 0.9uL water
- 0.1uL PrepGEM substrate

---Revised (as of 4/30/10)---

Per reaction:
- 0.1uL 10X Gold Buffer
- 0.89uL water
- 0.01uL PrepGEM substrate


Note: In order to make a reasonable mixture, enough volume for at least 10 reactions should be mixed at once and split up as much as needed.