Kun:LabNotes/CpgSeq/2008-3-18

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Revision as of 01:13, 19 March 2008 by KunZhang (talk | contribs) (New page: ==Exp. 1 Cpg capture with a new batch of bisulfite treated Jurkat DNA from 03/17/08== Sigma x2 Zymo x 2 Template 7 3 10X buffer(fre...)
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Exp. 1 Cpg capture with a new batch of bisulfite treated Jurkat DNA from 03/17/08

                      Sigma x2     Zymo x 2
  Template              7            3
  10X buffer(fresh)     1            1
  Cpg28k probe(218nM)   2            2
  H2O                   0            4

Exon capture control

  GM20431 131ng/ul      7          
  10X buffer(fresh)     1           
  E55kv1 probe(220nM)   2           
  H2O                   0            

94C 10min -> 60C 10min -> add 1ul NSL mix (Stoffel 2U/ul, Ampligase 0.5U/ul, dNTP 10nM or 50uM) -> 60C 1h -> 59 cycles of (94C 1min -> 60C 1h) -> 60C hold -> 37C 1min -> add 2ul Exo I&III, 37C 2h -> 94C 5min -> 4C hold.