Kun:LabNotes/CpgSeq/2010-6-8
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Probe prep for the DMR220k set
Library name OLID Part # DMR220k_a 0284601 G4446A DMR220k_b 0284591 G4446A DMR220k_c 0284581 G4446A DMR220k_d 0284571 G4446A
- Resuspend the oligos in each tube with 500ul nuclease-free H2O.
Expansion PCR
x 4 DMR220k_a (20nM) 1ul DMR220k_b (20nM) 1ul DMR220k_c (20nM) 1ul DMR220k_d (20nM) 1ul 2x iTaq master mix: 100ul 100uM AP1V6: 0.8ul 100uM AP2V6: 0.8ul H2O: 96ul 94C 2min -> (94C 30sec -> 54C 45sec -> 72C 45sec) x 16 -> 72C 3min -> 15C hold Purify the amplicons with two Qiaquick columns, eluted with 40ul EB.