AlanFung:LabNotes/ASE/2010/2010-9-13
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Analysis of the Hues6/BJ hybrid data
Summary of experiments
Index Table
- tmp=HL071
- tmp2=HL068_2
gDNA | cDNA | |||||
HL068_2 | HL071 | HL068_2 | HL071 | |||
5+6 | 5 + 6 | 7+8 | 7+8 | |||
IND1 | N/A | H1 gDNA | IND1 | 1000s | 1000s | |
IND2 | 1000ips | 1000ips | IND2 | 1000ips | 1000ips | |
IND3 | 1034ips | 1034ips | IND3 | 1034ips | 1034ips | |
IND4 | A2 | A2 | IND4 | A2 | A2 | |
IND5 | B4 | B4 | IND5 | B4 | B4 | |
IND6 | 1016ips | 1016ips | IND6 | 1016s | 1016s | |
IND7 | N/A | N/A | IND7 | 1016ips | 1016ips | |
IND8 | B5 | B5 | IND8 | B5ips | B5ips | |
IND9 | Hues6 | Hues6 | IND9 | Hues6 | Hues6 | |
IND10 | N/A | N/A | IND10 | BJ | BJ | |
IND11 | BJ | BJ | IND11 | N/A | 1034s | |
IND12 | N/A | H1 cDNA | IND12 | N/A | N/A | |
Wiki | KZ 7_22 | HL 8_26 | HL 7_26 | HL 8_26 |
Workflow
1) Convert using BCL converter
2)Demultiplex Script for demultiplex
3)Combine files Use Excel to create script for merging ID filles from serval lanes of Data to one file Create separate files for all samples make directory script Merge 68 and 71 script
4)Run aseBowtieSam.pl
5)Run baseCalls2Ase.pl on local machine