Sam:LabNotes/Microbiome-new/2010-9-24
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Sanger sequencing test on E.coli gDNA MDA amplicons
Background
- To optimize the Sanger sequencing protocl for MDA amplicon validation
- This protocol will also include the S1 nucelase debranching, DNA Pol.I reparing procedures, and compare the difference between with and without these treatments.
Procedures
- Sample: E.coli gDNA MDA amplicons made on 09-10-'10 (666ng/uL)
- S1 nuclease digestion (debranching)