Sam:LabNotes/Microbiome-new/2010-11-15

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Revision as of 08:03, 16 November 2010 by >Sam Chiang (→‎Procedures)
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2nd size selection of pooled Illumina library(short and long amplified E.coli SAGs -3rd test 11-07-'10)

Background

Procedures

  • The pooled library is ~40uL. The pooled library was seperated into two tube (20uL/tube) for size-selection using 5-well TBE-PAGE.
    • Sample well: 10uL H2O + 10uL 6X loading dye + 20uL DNA sample
    • Ladder well: 30uL H2O + 10uL 6X loading dye + 0.5uL Low mass ladder/1kb plus ladder
  • Run at 200V for 30min. Long staining (15min with foil wrap protection).
File:Sam111510-TBE PAGE.jpg  File:Sam111510-TBE PAGE-post cut.jpg