Rui Liu
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Lab Projects
Lab Meetings
Manuals
Zeroblunt cloning
Bio-Rad QPCR machine
- Program on desktop: "Opticon Moniter 3"
- Instrument: Chromo4:CD003042. "Instrument"-->"Scan for instruments" or "Quick load"(under "Master")-->choose "Chromo4:CD003042"
- "Prepare new run" or "open" a old file and "edit"
- "Plate setup"-->"edit"-->select "samples" and "SBG1" for SYBR green I
- "Protocol setup"-->"edit"-->add "plate read" before "repeat cycle"
- "Melting curve" optional, hold at 15C, volume 20ul
- "Run" and "Save", files are saved in "Opticon Users 3"/Rui
- "Status" and "Quantitation" are used for monitoring PCR process
- "Quantitation"-->"copy to clipboard"-->"Data graph" and edit under "Paint"
Ep Realplex QPCR machine
- "Mastercycler ep realplex" on desktop
- log in User name: "EPPENDORF", password: "e"
- "PCR program"-->direct change temp and time-->right click to insert and advance edit
- "Plate layout"-->"Filer 520nm"-->"SYBR"; "Sample volume", "Probe"-->"SYBR green", "Background"-->"Biorad strip tube-50ul" (Attention!); select sample area--> right click-->"unknown"-->ID
- "Save as" a "template" or an "assay"
- "Set up"-->"System Configuration"-->"Cycler"-->"Lid"-->select "Heat"-->Okey and close for preheat the lid
- "Monitoring" for PCR process
- "Analysis data" and save as assay