Rui Liu

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Revision as of 20:40, 28 February 2011 by >Zsakura2 (→‎Zeroblunt cloning)
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Lab Projects

Lab Meetings

Manuals

Zeroblunt cloning

TA cloning

Vector map [1]

Bio-Rad QPCR machine

  1. Program on desktop: "Opticon Moniter 3"
  2. Instrument: Chromo4:CD003042. "Instrument"-->"Scan for instruments" or "Quick load"(under "Master")-->choose "Chromo4:CD003042"
  3. "Prepare new run" or "open" a old file and "edit"
  4. "Plate setup"-->"edit"-->select "samples" and "SBG1" for SYBR green I
  5. "Protocol setup"-->"edit"-->add "plate read" before "repeat cycle"
  6. "Melting curve" optional, hold at 15C, volume 20ul
  7. "Run" and "Save", files are saved in "Opticon Users 3"/Rui
  8. "Status" and "Quantitation" are used for monitoring PCR process
  9. "Quantitation"-->"copy to clipboard"-->"Data graph" and edit under "Paint"

Ep Realplex QPCR machine

  1. "Mastercycler ep realplex" on desktop
  2. log in User name: "EPPENDORF", password: "e"
  3. "PCR program"-->direct change temp and time-->right click to insert and advance edit
  4. "Plate layout"-->"Filer 520nm"-->"SYBR"; "Sample volume", "Probe"-->"SYBR green", "Background"-->"Biorad strip tube-50ul" (Attention!); select sample area--> right click-->"unknown"-->ID
  5. "Save as" a "template" or an "assay"
  6. "Set up"-->"System Configuration"-->"Cycler"-->"Lid"-->select "Heat"-->Okey and close for preheat the lid
  7. "Monitoring" for PCR process
  8. "Analysis data" and save as assay

Microscope