November5, 2010 (Mouse gDNA from La Spada's lab, UCSD)
received 8 gDNA samples from Paula Ladd
Samples
Sample ID
concentration (ng/ul)
volume (ul)
1. wild-type cortex
WT-CTX
2000.0
3.8
2. wild-type cerebellum
WT-CBL
100.0
8.2
3. wild-type brain stem
WT-BS
600.0
8.5
4. wild-type retina
WT-RET
1800.0
4.0
5. transgenic cortex
TG-CTX
250.0
17.0
6. transgenic cerebellum
TG-CBL
400.0
14.0
7. transgenic brain stem
TG-BS
1000.0
6.5
8. transgenic retina
TG-RET
2000.0
8.0
Note: WT: wild-type, TG: transgenic
Samples were diluted to 200ng/ul based on original concentration from Paula and confirmed by Nanodrop
Samples
Sample ID
total volume (ul) after diluted
concentration(ng/ul)
1. wild-type cortex
WT-CTX
25.0
223.2
2. wild-type cerebellum
WT-CBL
25.0
663.5
3. wild-type brain stem
WT-BS
25.0
86.0
4. wild-type retina
WT-RET
25.0
163.7
5. transgenic cortex
TG-CTX
20.0
219.2
6. transgenic cerebellum
TG-CBL
25.0
109.4
7. transgenic brain stem
TG-BS
25.0
103.0
8. transgenic retina
TG-RET
25.0
126.6
labnote: 2010_11_08
December 7, 2010 (Sarah's African gDNA, UPenn )
received 48 methylome samples from Tishkoff's lab delivered by UPS
William R Beggs, Jr. Tishkoff Lab - Dept of Genetics, University of Pennsylvania 430 Clinical Research Building 415 Curie Blvd Philadelphia, PA 19104-6145
LabID
1M sample #
Well
Volume ul
Conc ng/ul approx
CAPB016
A01
50
100
CAPB046
B01
50
100
CAPB056a
C01
50
100
CAPL056
D01
50
100
CAPM007
E01
50
100
TZHZ018A
F01
50
100
TZHZ033B
G01
50
100
TZHZ075A
H01
50
100
TZHZ214
A02
50
100
TZHZ221B
B02
50
100
TZSW067
C02
50
100
TZSW128B
D02
50
100
TZSW131A
E02
50
100
TZSW132B
F02
50
100
TZSW135B
G02
50
100
CAMF013A
H02
50
100
CAFU043
A03
50
100
CAFU042
B03
50
100
CAFU028
C03
50
100
CAMF022A
D03
50
100
CAPB043
E03
50
100
CAPL049
F03
50
100
CAPM001A
G03
50
100
CAPL036
H03
50
100
KEBR007A
A04
50
100
KEBR028A
B04
50
100
KEBR042B
C04
50
100
KEBR040A
D04
50
100
KEBR061A
E04
50
100
KEPK003A
F04
50
100
KEPK006A
G04
50
100
KEPK007A
H04
50
100
KEPK010A
A05
50
100
KEPK016A
B05
50
100
CAPM047
son
C05
50
100
CAPM003A
parent
D05
50
100
CAPM004A
parent
E05
50
100
CAPM056A
daughter
F05
50
100
ETAM042
G05
50
100
ETAM071
H05
50
100
ETAM077
A06
50
100
ETAM058
B06
50
100
ETAM065
C06
50
100
ETSB008
D06
50
100
ETSB027
E06
50
100
ETSB031
F06
50
100
ETSB035
G06
50
100
ETSB036
H06
50
100
aliquot 21ul out of 50ul samples into another 96-well plate
Note: Since the capture of the GA plate1 failed, and I needed to repeated the experiment, Ling brought the new plate (called GA plate1-2) with the same samples to me and all concentration were measured again by Nanodrop.
Note: Since I thought that they all are brain tissue samples, some labeling of library name or images used brain instead of blood. To prevent confusing, every labeling should be changed.
August25, 2011, gDNA from fibroblast cells that are the somatic line of the three single nonsynonymous mutation iPS on the record of July29, 2011
Received 2 gDNA samples from fibroblast cells that are the somatic lines of the three single nonsynonymous mutation iPS from Alice. Both of them were from the first elution in DNA purification
Sample ID
Conc. (ng/ul) by Sergio
Conc. (ng/ul)confirmed by Nanodrop
260/280
260/230
1. 2HFF p6
283.50
280.10
2.02
1.87
2. 218 HFF p7
586.30
446.10
2.05
2.00
Related lab note:
September16, 2011, gDNA from Sergio, Salk Institute
Sample ID
Conc. (ng/ul) by Sergio
Conc. (ng/ul)confirmed by Nanodrop
260/280
260/230
1. H9 P43 mTeSR
62.00
62.80
1.89
0.99
2. H9 p43+Activin 100ng/mL 5days
90.80
90.80
1.85
0.70
3. H9 p43+BMP4 50ng/mL 5days
91.10
93.70
1.85
1.15
4. HUViPS4F1 p19
95.20
98.00
1.84
1.22
5. HUViPS4F1 p19+Activin 100ng/mL 5days
83.80
84.80
1.79
0.78
6. HUViPS4F1 p19+BMP4 50ng/mL 5days
80.00
78.60
1.84
0.96
7. HUViPS4F3
96.80
96.90
1.87
1.12
8. HUViPS4F3+Activin 100ng/mL 5days
90.20
91.90
1.78
0.80
9. HUViPS4F3+BMP4 50ng/mL 5days
80.20
80.60
1.86
0.96
Related lab note:
September16, 2011, received 3 more gDNA from UPenn to repeat the capture of the samples with low QC values
Sample ID
Conc. (ng/ul) by Sergio
Conc. (ng/ul)confirmed by Nanodrop
260/280
260/230
1. KEBR028A
50.00
23.80
1.83
0.51
2. TZHZ031A
50.00
55.10
1.88
1.78
3. TZHZ041A
50.00
54.60
1.88
1.35
Related lab note:
September 17, 2011), received 3 cell pellet samples from Alice (sample from Yang Xu's lab)
I extracted gDNA and elutee 150ul in elute1 and elute2. I concentration was was measure by Nanodrop.