Sam:LabNotes/Microbiome-new/2010-12-8

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Revision as of 18:44, 13 December 2010 by >Sam Chiang (Created page with '='''Data analysis of E.coli SAGs sequencing results(short and long amplified E.coli SAGs -3rd test 11-07-'10)'''= ==Background== *Eight E.coli SAGs samples were made to Illumina…')
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Data analysis of E.coli SAGs sequencing results(short and long amplified E.coli SAGs -3rd test 11-07-'10)

Background

  • Eight E.coli SAGs samples were made to Illumina sequencing libraries using (N2)Primer-based barcodes ID1 to ID8.
  • These libraries were equally pulled (20ng per library) and sequenced in pair-end(PE) mode.
  • The sequenced data processed by Dr. Zhang for base-calling and saved into FASTQ files.
  • Since I don't have genome-tech server permission, I asked Dinh's help to transfer data into Genemapster server.

Analysis approaches

  • Step1. Performing whole-read mapping using b