Rui:SingelCell

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Revision as of 20:39, 9 January 2011 by >RuiLiu (→‎Background)
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Background

  • Goal: the smallest functional unit; the exact quantity; full-length RNAs; sense vs anti-sense; all classes; at single-base resolution
  • Step 1: single cell; exact quantity; the 3' end; mRNA; RNAseq

Protocols

PCR-based protocol

IVT-based protocol

Circulation-based protocol

2011

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