Rui:LabNotes/SingleCell/2011-1-20
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1. Measure the concentration of the RNA samples
- Hues 54 414.50ng/ul 2.06 2.01
- Hues6-ES 641.13ng/ul 2.12 2.11
- Hues6-EB 561.99ng/ul 2.11 2.11
2. Examine the integrity of the RNA samples
- 100ml agarose gel + 8ul SYBR safe
3. Dilute RNA samples in PCR 8-tube strip (-80C)
- 1) 1:10
- 2) 1:10
- 3) 1:4/1:6/1:5.6 --> 1ng/ul
- 4) 1:5 --> 0.2ng/ul
- 5) 1:10 --> 0.02ng/ul