Rui:LabNotes/SingleCell/2011-1-20

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Revision as of 20:54, 20 January 2011 by >RuiLiu (Created page with '1. Measure the concentration of the RNA samples *Hues 54 414.50ng/ul 2.06 2.01 *Hues6-ES 641.13ng/ul 2.12 2.11 *Hues6-EB 561.99ng/ul 2.11 2.11 2. Examine the int…')
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1. Measure the concentration of the RNA samples

  • Hues 54 414.50ng/ul 2.06 2.01
  • Hues6-ES 641.13ng/ul 2.12 2.11
  • Hues6-EB 561.99ng/ul 2.11 2.11

2. Examine the integrity of the RNA samples

  • 100ml agarose gel + 8ul SYBR safe

3. Dilute RNA samples in PCR 8-tube strip (-80C)

  • 1) 1:10
  • 2) 1:10
  • 3) 1:4/1:6/1:5.6 --> 1ng/ul
  • 4) 1:5 --> 0.2ng/ul
  • 5) 1:10 --> 0.02ng/ul