Rui:LabNotes/SingleCell/2011-1-27

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Characterization of positive control

*Pure cDNA w/o primer carry-over
*1st round of AMpure beads purification, 20ul --> 36ul beads (1.8*) --> 36ul water
*2nd round of AMpure beads purification, 30ul --> 21ul beads (0.7* suggested by Alice) --> 36ul water
Concentration   Nanodrop [ng/ul]       Qubit [ug/ml]
1st Hues54      38.67 (2.16; 2.07)     3.76
    Hues6-ES    58.78 (2.11; 2.27)     6.05
    Hues6-EB    55.25 (2.07; 2.04)     6.54
2nd Hues54      19.72 (2.36; 2.35)     1.29
    Hues6-ES    40.1  (2.19; 2.24)     2.44
    Hues6-EB    35.92 (2.21; 2.14)     2.64

Titration of Exonuclease I

*Goal: high enough to remove primer/unspecific amplification on NTC; low enough to avoid interference to cDNA stability and following steps
*Positive control: diluted cDNA w/o primer
*Negative control: Rui water
*Additional note: ds DNA treatment for single cell???