Rui:Haplotying

From ZhangLabWiki
Revision as of 23:23, 14 November 2011 by >RuiLiu (→‎Protocol)
Jump to navigation Jump to search

Progress

Sequencing analysis

  • Dr. Zhang's link on haplotyping analysis [1]

Notes

  • "Methylation haplotyping on few cells" project: May 27th email [5]
  • Human gDNA samples: Kun's stack - the box at the left up corner (-20C), Rui's box (4C) with GC DNA and Africa's DNA.
  • Prepare ABD mixture: 9 mL 40% acrylamide, 1 ml Acrylamide/Bis (19:1), 200 mg DATD in 15 mL tube. Shake to dissolve DATD. Use a 3 cc syringe and .22 um filter to 1 mL of ABD into 1.5 mL tube. The rest can be saved at 4C for 1 week

Protocol

Primers

File:Primers for Tn-based-haplotyping.xls

2011

<calendar> name=Rui:LabNotes/Haplotyping format=%name/%year-%month-%day date=2011/10/1 view=oneyear </calendar>