Noi/NOTES/2011-2-15

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Revision as of 18:52, 17 February 2011 by >Noi
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DMR220k capture(Probe set S1-S5 plus new probe set Exp1-Exp3) and library construction of Parkinson's patients

  • Received 18 human brain samples from Pakinson's patients. This includes control and Pakinson's patiants (2011_02_02)
  • Human frozen cerebral cortex
Pair Control Sample ID Sample code Sex age (year.day) Postmortem time Conc. (ug/ul)/ volume (ul) PD Sample ID Sample code Sex
1. 5028 P1C M 67.29 P18 0.5ug/ul/ 4ul 1947 P1P M
2. 4789 P2C F 72.05 P19 0.5ug/ul/ 4ul 4977 P2P F
3. 5171 P3C M 79.09 P05 0.5ug/ul/ 4ul 4879 P3P M
4. 1818 P4C M 76.29 P03 0.5ug/ul/ 4ul 4526 P4P M
5. 5089 P5C M 89.02 P14 0.5ug/ul/ 4ul 5203 P5P M
6. 5237 P6C M 52.29 P13 0.5ug/ul/ 4ul 1910 P6P M
7. 1569 P7C1 F 77.09 P08 0.5ug/ul/ 4ul 1401 P7P F
7. 5219 P7C2 F 76.35 P03 0.5ug/ul/ 4ul
8. 4735 P8C M 73.18 P21 0.5ug/ul/ 4ul 1741 P8P1 M
8. 5306 P8P2 M


Bisulfite conversion

  • gDNA total volume 4ul (0.5ng/ul) so total amount = 2ug
  • Add EB 6 ul to make volume to 10ul, so the final concentration should be 200ng/ul
Sample ID Conc.(ng/ul) Volume (ul) H20 (ul) CT conversion reagent (ul) Total volume (ul)
P1C 200.00 5.00 15.00 130.00 150.00
P2C 200.00 5.00 15.00 130.00 150.00
P3C 200.00 5.00 15.00 130.00 150.00
P4C 200.00 5.00 15.00 130.00 150.00
P5C 200.00 5.00 15.00 130.00 150.00
P6C 200.00 5.00 15.00 130.00 150.00
P7C1 200.00 5.00 15.00 130.00 150.00
P7C2 200.00 5.00 15.00 130.00 150.00
P8C 200.00 5.00 15.00 130.00 150.00
P1P 200.00 5.00 15.00 130.00 150.00
P2P 200.00 5.00 15.00 130.00 150.00
P3P 200.00 5.00 15.00 130.00 150.00
P4P 200.00 5.00 15.00 130.00 150.00
P5P 200.00 5.00 15.00 130.00 150.00
P6P 200.00 5.00 15.00 130.00 150.00
P7P 200.00 5.00 15.00 130.00 150.00
P8P1 200.00 5.00 15.00 130.00 150.00
P8P2 200.00 5.00 15.00 130.00 150.00


  • follow standard protocol for bisulfite conversion of ZymoResearch
  • elute with 17ul Elution buffer
  • perform