Noi/NOTES/2011-2-15
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DMR220k capture(Probe set S1-S5 plus new probe set Exp1-Exp3) and library construction of Parkinson's patients
- Received 18 human brain samples from Pakinson's patients. This includes control and Pakinson's patiants (2011_02_02)
- Human frozen cerebral cortex
Pair | Control Sample ID | Sample code | Sex | age (year.day) | Postmortem time | Conc. (ug/ul)/ volume (ul) | PD Sample ID | Sample code | Sex |
1. | 5028 | P1C | M | 67.29 | P18 | 0.5ug/ul/ 4ul | 1947 | P1P | M |
2. | 4789 | P2C | F | 72.05 | P19 | 0.5ug/ul/ 4ul | 4977 | P2P | F |
3. | 5171 | P3C | M | 79.09 | P05 | 0.5ug/ul/ 4ul | 4879 | P3P | M |
4. | 1818 | P4C | M | 76.29 | P03 | 0.5ug/ul/ 4ul | 4526 | P4P | M |
5. | 5089 | P5C | M | 89.02 | P14 | 0.5ug/ul/ 4ul | 5203 | P5P | M |
6. | 5237 | P6C | M | 52.29 | P13 | 0.5ug/ul/ 4ul | 1910 | P6P | M |
7. | 1569 | P7C1 | F | 77.09 | P08 | 0.5ug/ul/ 4ul | 1401 | P7P | F |
7. | 5219 | P7C2 | F | 76.35 | P03 | 0.5ug/ul/ 4ul | |||
8. | 4735 | P8C | M | 73.18 | P21 | 0.5ug/ul/ 4ul | 1741 | P8P1 | M |
8. | 5306 | P8P2 | M |
Bisulfite conversion
- gDNA total volume 4ul (0.5ng/ul) so total amount = 2ug
- Add EB 6 ul to make volume to 10ul, so the final concentration should be 200ng/ul
Sample ID | Conc.(ng/ul) | Volume (ul) | H20 (ul) | CT conversion reagent (ul) | Total volume (ul) |
P1C | 200.00 | 5.00 | 15.00 | 130.00 | 150.00 |
P2C | 200.00 | 5.00 | 15.00 | 130.00 | 150.00 |
P3C | 200.00 | 5.00 | 15.00 | 130.00 | 150.00 |
P4C | 200.00 | 5.00 | 15.00 | 130.00 | 150.00 |
P5C | 200.00 | 5.00 | 15.00 | 130.00 | 150.00 |
P6C | 200.00 | 5.00 | 15.00 | 130.00 | 150.00 |
P7C1 | 200.00 | 5.00 | 15.00 | 130.00 | 150.00 |
P7C2 | 200.00 | 5.00 | 15.00 | 130.00 | 150.00 |
P8C | 200.00 | 5.00 | 15.00 | 130.00 | 150.00 |
P1P | 200.00 | 5.00 | 15.00 | 130.00 | 150.00 |
P2P | 200.00 | 5.00 | 15.00 | 130.00 | 150.00 |
P3P | 200.00 | 5.00 | 15.00 | 130.00 | 150.00 |
P4P | 200.00 | 5.00 | 15.00 | 130.00 | 150.00 |
P5P | 200.00 | 5.00 | 15.00 | 130.00 | 150.00 |
P6P | 200.00 | 5.00 | 15.00 | 130.00 | 150.00 |
P7P | 200.00 | 5.00 | 15.00 | 130.00 | 150.00 |
P8P1 | 200.00 | 5.00 | 15.00 | 130.00 | 150.00 |
P8P2 | 200.00 | 5.00 | 15.00 | 130.00 | 150.00 |
- follow standard protocol for bisulfite conversion of ZymoResearch
- elute with 17ul Elution buffer
- perform