Calendar/2011-3-28

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Revision as of 19:53, 28 March 2011 by >Alan6017518 (Created page with '==Library Construction on sample 1-21 from Dr. Heller== ===Qubit Quantification=== ===Tagmentation Reaction=== *Prior to assembling the reaction, briefly centrifuge the 5X Next…')
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Library Construction on sample 1-21 from Dr. Heller

Qubit Quantification

Tagmentation Reaction

  • Prior to assembling the reaction, briefly centrifuge the 5X Nextera Reaction Buffer and Nextera Enzyme Mix tubes to assure that the reagents are at the bottom of the tubes.
  • Assemble the following reaction components on ice, in the order listed:
x µl Nuclease-Free Water 
50 ng Target DNA (in T10E1 Buffer [10 mM Tris-HCl (pH 7.5), 1 mM EDTA])
4 µl 5X Nextera Reaction Buffer LMW or HMW (see Important Considerations, no. 1, p. 3)
1 µl Nextera Enzyme Mix (Illumina-compatible)
20 µl Total reaction volume