Calendar/2011-3-28

From ZhangLabWiki
Revision as of 20:16, 28 March 2011 by >Alan6017518 (→‎Qubit Quantification)
Jump to navigation Jump to search

Library Construction on sample 1-21 from Dr. Heller

Qubit Quantification

  • dilute 25ul 200X dye with 4975ul buffer

Tagmentation Reaction

  • Prior to assembling the reaction, briefly centrifuge the 5X Nextera Reaction Buffer and Nextera Enzyme Mix tubes to assure that the reagents are at the bottom of the tubes.
  • Assemble the following reaction components on ice, in the order listed:
x µl Nuclease-Free Water 
50 ng Target DNA (in T10E1 Buffer [10 mM Tris-HCl (pH 7.5), 1 mM EDTA])
4 µl 5X Nextera Reaction Buffer LMW or HMW (see Important Considerations, no. 1, p. 3)
1 µl Nextera Enzyme Mix (Illumina-compatible)
20 µl Total reaction volume